abstract：:A variety of transcription attenuation mechanisms are used by bacteria to regulate gene and operon expression. This review summarizes previous and current studies designed to elucidate the features of the specific attenuation mechanisms that regulate expression of the tryptophanase (tna) operon of Escherichia coli and...

journal_title：Biochimie

authors： Yanofsky C,Konan KV,Sarsero JP

更新日期：1996-01-01 00:00:00

abstract：:Influenza virus-associated RNA polymerase is composed of one molecule each of three viral P proteins and carries the complete activity of capped RNA-primed vRNA-directed transcription. The RNA polymerase holoenzyme also carries capped RNA endonuclease to generate capped oligonucleotide primers for transcription and 3'...

journal_title：Biochimie

authors： Ishihama A

更新日期：1996-01-01 00:00:00

abstract：:Two soluble forms of brain glutamate dehydrogenase isoproteins were inactivated by pyridoxal 5'-phosphate. Restoration of catalytic activity can be accomplished by dialysis and addition of an excess of cysteine or lysine. Spectral evidence is presented to indicate that the inactivation proceeds through Schiff base for...

journal_title：Biochimie

authors： Cho SW,Lee JE

更新日期：1996-01-01 00:00:00

abstract：:Although halogenated hydrocarbons are noted for low chemical reactivity, small amounts are toxic to humans. Cytochromes P450 have been implicated in transforming these compounds to more reactive species, under anaerobic conditions, through reduction at the heme. A significant amount of effort has been directed toward ...

journal_title：Biochimie

authors： Manchester JI,Ornstein RL

更新日期：1996-01-01 00:00:00

abstract：:Previous work from this laboratory has shown that T7 RNA polymerase outpaces ribosomes in vivo, generating naked mRNA stretches which may be nuclease-sensitive. In particular, lacZ transcripts synthesised this way are highly unstable and yield little beta-galactosidase. We have argued that most of these transcripts ar...

journal_title：Biochimie

authors： Lopez PJ,Dreyfus M

更新日期：1996-01-01 00:00:00

abstract：:The term 'RNA editing' was used for the first time in 1986 to describe the process of uridylate insertion into trypanosomal mitochondrial transcripts. Since then, the term has been used more generally to describe a large variety of processes involving base insertions, deletions and conversions that generate RNAs with ...

journal_title：Biochimie

authors： Blanc V,Jordana X,Litvak S,Araya A

更新日期：1996-01-01 00:00:00

abstract：:The effects of various concentrations of 2-fluoro-2-deoxy-D-glucose (FDG) on the aerobic metabolism of glucose and the reciprocal effect of glucose on the metabolism of FDG in glucose-grown repressed Saccharomyces cerevisiae cells were studied at 30 degrees C in a standard pyrophosphate medium containing 5 x 10(7) cel...

journal_title：Biochimie

authors： Tran-Dinh S,Courtois A,Wietzerbin J,Bouet F,Herve M

更新日期：1995-01-01 00:00:00

abstract：:The suhB gene of Escherichia coli has been defined by its mutant allele that suppresses other mutants in secY, rpoH, dnaB, and era. The suhB mutant by itself is cold sensitive, and is shown to have defects in protein synthesis. Starting with the suhB cold-sensitive mutant, cold-resistant suppressors were isolated. The...

journal_title：Biochimie

authors： Inada T,Nakamura Y

更新日期：1995-01-01 00:00:00

abstract：:Cytochromes P450 constitute a superfamily of hemoproteins which have evolved from a common ancestor. Recent advances in molecular biology have offered a powerful approach to the research on the multiplicity of P450. Now, every mammalian P450 species can be characterized by heterologous expression of its cDNA. In addit...

journal_title：Biochimie

authors： Yabusaki Y

更新日期：1995-01-01 00:00:00

abstract：:Wheat germ lysate was used as a model system for in vitro translation. We show that an increase of the exchange surface between the reaction mixture and the atmosphere enhanced the amount of incorporated cysteine, indicating that early arrest of protein synthesis previously observed in such a system was due to oxygen ...

journal_title：Biochimie

authors： Luciani N,Hess K,Belleville F,Nabet P

更新日期：1995-01-01 00:00:00

abstract：:Genetic elements coding for proteins that present amino acid identity with the conserved motifs of retroviral reverse transcriptases constitute the retroid family. With the exception of reverse transcriptases encoded by mitochondrial plasmids of Neurospora, all reverse transcriptases have an absolute requirement for a...

journal_title：Biochimie

authors： Marquet R,Isel C,Ehresmann C,Ehresmann B

更新日期：1995-01-01 00:00:00

abstract：:A nuclear poly(ADP-ribose) polymerase (PARP) is activated by gamma-irradiation and consequently synthesizes poly(ADP-ribose) by binding to DNA strand-breaks. This property suggests that PARP is a DNA strand-break-signal generator. Meanwhile, the cell-cycle arrest occurs in G1 and G2 phases following gamma-irradiation....

journal_title：Biochimie

authors： Masutani M,Nozaki T,Wakabayashi K,Sugimura T

更新日期：1995-01-01 00:00:00

abstract：:We have analyzed the interference of antitumoral drugs acting through the inhibition of DNA topoisomerase II on the human HeLa cell metabolism. Different compounds characterized by a diverse mechanism of action have been used, namely m-amsacrine, an intercalative drug, etoposide, which does not intercalate DNA, and su...

journal_title：Biochimie

authors： Negri C,Bernardi R,Donzelli M,Scovassi AI

更新日期：1995-01-01 00:00:00

abstract：:The very end of the chromosome is called the telomere and is composed of DNA repeat sequences and associated proteins. Genetic and biochemical analyses of this complex, the telosome, lead to the hypothesis that transcription and DNA replication are submitted to position effects mediated by the telomere proximity. Telo...

journal_title：Biochimie

authors： Pommier JP,Lebeau J,Ducray C,Sabatier L

更新日期：1995-01-01 00:00:00

abstract：:The study of modified nucleoside contributions to RNA chemistry, structure and function has been thwarted by the lack of a site-selected method of incorporation which is both versatile and adaptable to present synthetic technologies. A reproducible and versatile site-selected incorporation of nine differently modified...

journal_title：Biochimie

authors： Agris PF,Malkiewicz A,Kraszewski A,Everett K,Nawrot B,Sochacka E,Jankowska J,Guenther R

更新日期：1995-01-01 00:00:00

abstract：:The lignan 8,8'-bis-(methylenedioxy)cinnamic acid (BMDCA) is a powerful competitive inhibitor (K1 = 2.0 microM) of the lignin peroxidase (LiP) from Phanerochaete chrysosporium and of the extracellular peroxidase of Phlebia radiata (I0.5 = 10 microM). BMDCA derivatives with the same double bond system also inhibited th...

journal_title：Biochimie

authors： Frías I,Trujillo JM,Romero J,Hernandez J,Pérez JA

更新日期：1995-01-01 00:00:00

abstract：:RNA editing alters genomically encoded cytidines to uridines posttranscriptionally in higher plant mitochondria. Most of these editing events occur in translated regions and consequently alter the amino acid sequence. In Oenothera berteriana more than 500 editing sites have been detected and the total number of editin...

journal_title：Biochimie

authors： Yu W,Fester T,Bock H,Schuster W

更新日期：1995-01-01 00:00:00

abstract：:RNA phosphodiester bonds can be cleaved by metal ions, of which Pb2+ is one of the most effective. It can cleave both generally and site-specifically, depending on the substrate and the conditions. In addition, metal ions are also known to cleave ester bonds between amino acid and the 3'-end of transfer RNA. Here we r...

journal_title：Biochimie

authors： Otzen DE,Barciszewski J,Clark BF

更新日期：1994-01-01 00:00:00

abstract：:Although a 30 kDa polypeptide frequently is seen by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of post mortem (pm) skeletal muscle and in turn is used as an indicator of proteolysis, its origin has not been conclusively identified. We used antibodies to selected myofibrillar proteins...

journal_title：Biochimie

authors： Ho CY,Stromer MH,Robson RM

更新日期：1994-01-01 00:00:00

abstract：:In order to identify conserved potential secondary structures within translational start sites, mRNA sequences derived from different species were studied with programs able to depict such features. The potential secondary structure of 71 bases around the initiator AUG or AUGs in the coding sequences of 290 eukaryotic...

journal_title：Biochimie

authors： Ganoza MC,Louis BG

更新日期：1994-01-01 00:00:00

abstract：:Rhodospirillum salexigens is a moderately halophilic purple phototrophic bacterium which grows optimally in 8% NaCl. The amino acid sequences of the two principal soluble cytochromes c have been determined. One of these is a cytochrome c2, similar in size to mitochondrial cytochrome c. While clearly of the same sequen...

journal_title：Biochimie

authors： Ambler RP,Daniel M,Meyer TE,Kamen MD

更新日期：1994-01-01 00:00:00

abstract：:This paper provides an overview of the usefulness of the resonance Raman (RR) spectroscopy in the determination of the structural and electronic properties of heme(s) included in c-type cytochromes. It reviews the mode assignments presently available for heme c and includes recent RR data on the most important subclas...

journal_title：Biochimie

authors： Desbois A

更新日期：1994-01-01 00:00:00

abstract：:The product of the sex-determining gene SRY is a member of the HMG box containing protein superfamily. The HMG box is a DNA-binding domain of about 80 amino acids shared by many proteins with diverse functions. It seems that the functions of the full length protein are restricted to the HMG box but their molecular bas...

journal_title：Biochimie

authors： Soullier S,Poulat F,Boizet-Bonhoure B,Calas B,Bennes R,Heitz F,Berta P

更新日期：1994-01-01 00:00:00

abstract：:The Escherichia coli Fis protein is a dimeric DNA-binding protein whose specific binding sites share a weak consensus sequence. Use of the gel retardation technique indicates that binding of Fis on a linear DNA fragment leads to the formation of a ladder of defined retarded complexes, independently of the presence of ...

journal_title：Biochimie

authors： Bétermier M,Galas DJ,Chandler M

更新日期：1994-01-01 00:00:00

abstract：:Isopentenyl adenosine derivatives can be found next to the anticodon (position 37) in tRNA from both the Bacteria and Eucarya domains. These modified nucleosides improve the efficiency of tRNA in translation, can increase and decrease translational fidelity, and make the tRNA less codon context sensitive. In bacteria ...

journal_title：Biochimie

authors： Persson BC,Esberg B,Olafsson O,Björk GR

更新日期：1994-01-01 00:00:00

abstract：:Structural studies on various electron transfer complexes involving the tetrahemic cytochrome c3 provided evidence that one of the hemes (heme 4) is the interacting site on the molecule. The reactivity of this particular heme is allocated to the positive charges found around the heme group which are strongly involved ...

journal_title：Biochimie

authors： Guerlesquin F,Dolla A,Bruschi M

更新日期：1994-01-01 00:00:00

abstract：:This review discusses the regulation of a key controlling step in the initiation of protein synthesis, the binding of mRNA to ribosomes. Particular focus is given to the phosphorylation of the cap-binding factor, eIF-4E, and the role of this factor in the regulation of cell growth. ...

journal_title：Biochimie

authors： Sonenberg N

更新日期：1994-01-01 00:00:00

abstract：:The structure difference between the free operator of the lac system d(GCTCACAAT).d(ATTGTGAGC) and the same operator complexed to the headpiece of the lac repressor has been investigated by 2-D-1H NMR spectroscopy in conjunction with molecular modelling in internal coordinates (JUMNA). The free and complexed operator ...

journal_title：Biochimie

authors： Gincel E,Lancelot G,Maurizot JC,Thuong NT,Vovelle F

更新日期：1994-01-01 00:00:00

abstract：:Many peptide hormone and neuropeptide precursors undergo post-translational processing at mono- and/or dibasic residues. An enzymatic activity capable of processing prodynorphin at a monobasic processing site designated 'dynorphin converting enzyme' has been previously reported in rat rain and bovine pituitary. In thi...

journal_title：Biochimie

authors： Berman YL,Rattan AK,Carr K,Devi L

更新日期：1994-01-01 00:00:00

abstract：:The specific binding of the amyloid protein precursor (APP) to glycosaminoglycans (GAG) suggests that APP is a cell adhesion molecule (CAM) and/or substrate adhesion molecule (SAM). In order to characterize this activity of APP in the brain at the molecular level, we have purified and characterized the major APP speci...

journal_title：Biochimie

authors： Multhaup G

更新日期：1994-01-01 00:00:00

abstract：:Amyloid beta protein (beta/A4) is deposited in senile plaques of patients with Alzheimer's disease. This protein is derived from a larger membrane-associated protein, termed amyloid precursor protein (APP). The constitutive processing of APP occurs at the central portion of beta/A4, resulting in the release of large N...

journal_title：Biochimie

authors： Shimokawa M,Nakamura K,Maruyama K,Tagawa K,Miyatake T,Sugita H,Ishiura S,Suzuki K

更新日期：1993-01-01 00:00:00

abstract：:Insulin stimulates protein synthesis in skeletal muscle of young animals and has been reported to exert similar effects on a variety of mammalian cell types in culture. However, with chick embryo fibroblasts we found that the extent of this effect was very sensitive to the culture conditions of the cells prior to the ...

journal_title：Biochimie

authors： Jeffrey IW,Pain VM

更新日期：1993-01-01 00:00:00

abstract：:The issue of whether histone H1 possesses specificity of binding to certain nucleotide sequences in DNA is of fundamental importance to the suggested role of the linker histone in the regulation of gene transcription. The purpose of the present study was to reinvestigate the specificity of binding of histone H1 to the...

journal_title：Biochimie

authors： Yaneva JN,Zlatanova JS

更新日期：1993-01-01 00:00:00

abstract：:In recent years an increasing number of inherited decreases in man has been identified in which there is an impairment of one or more peroxisomal functions. Sofar 15 different peroxisomal disorders have been identified which can be subdivided into three distinct groups depending upon whether there is a generalized (gr...

journal_title：Biochimie

authors： Wanders RJ,Schutgens RB,Barth PG,Tager JM,van den Bosch H

更新日期：1993-01-01 00:00:00

abstract：:The class I glutamine (Gln) tRNA synthetase interacts with the anticodon and acceptor stem of glutamine tRNA. RNA hairpin helices were designed to probe acceptor stem and anticodon stem-loop contacts. A seven-base pair RNA microhelix derived from the acceptor stem of tRNA(Gln) was aminoacylated by Gln tRNA synthetase....

journal_title：Biochimie

authors： Wright DJ,Martinis SA,Jahn M,Söll D,Schimmel P

更新日期：1993-01-01 00:00:00

abstract：:A 3-D model of the tyrosylable tRNA-like domain of the genomic brome mosaic virus RNAs was built by computer modelling based on solution probing of the molecule with different chemical and enzymatic reagents. This model encompasses four major structural domains, including two peculiar substructures oriented perpendicu...

journal_title：Biochimie

authors： Felden B,Florentz C,Westhof E,Giegé R

更新日期：1993-01-01 00:00:00

abstract：:We present an approach for designing a knowledge-based system, called Sequence Acquisition In Context (SAIC), that will be able to cooperate with a biologist in the analysis of DNA sequences. The main task of the system is the acquisition of the expert knowledge that the biologist uses for solving ambiguities from gel...

journal_title：Biochimie

authors： Brézillon PJ,Zaraté P,Saci F

更新日期：1993-01-01 00:00:00

abstract：:The effects of DNA oligonucleotides complementary to potato spindle tuber viroid (PSTVd) on viroid infection were investigated. The oligonucleotides were used to form hybrids with PSTVd in the infection mixture. A 75% reduction of viroid infection was found when an oligonucleotide complementary to nucleotides 79-110 o...

journal_title：Biochimie

authors： Matousek J,Trnĕná L,Arnold L,Dĕdic P,Rakouský S,Steger G,Riesner D

更新日期：1993-01-01 00:00:00

abstract：:In order to confirm the presence of a phosphoenolpyruvate (PEP)-dependent sugar-phosphotransferase system (PTS) in the mollicute Acholeplasma florum we studied the ability of cell free extracts of this organism to phosphorylate glucose and/or fructose in the presence of PEP. We also cloned and sequenced a DNA fragment...

journal_title：Biochimie

authors： Navas-Castillo J,Laigret F,Hocquellet A,Chang CJ,Bove JM

更新日期：1993-01-01 00:00:00

abstract：:After treatment of L929 cells, a murine fibrosarcoma line, with tumor necrosis factor (TNF), a nuclear kappa B-like transcription factor is rapidly induced as identified by gel shift mobility assays using the kappa B-responsive sequence of the immunoglobulin or interleukin-6 (IL-6) genes as a DNA probe. When induction...

journal_title：Biochimie

authors： Patestos NP,Haegeman G,Vandevoorde V,Fiers W

更新日期：1993-01-01 00:00:00